Agilent Cell Analysis Publication Databaseīrowse the full list of publications using Agilent Cell Analysis data.Agilent Seahorse Assay Guides and Templates.Please consult the Agilent Cell Analysis Publication Database and/or the XF Publication database to provide an initial starting point for cell density values specific to your needs. Generally, densities resulting in 50-90% confluency generate metabolic rates in the desirable/dynamic range of the instrument. Suspension cells require higher seeding density from 5 x 10 4 to 2 x 10 5 cells per well depending on cell type. Optimal cell seeding number varies by cell type, but is typically between 5 x 10 3 and 4 x 10 4 cells per well for adherent cells. The Seahorse XF Real-Time ATP rate assay Test Kit The Seahorse XFp Real-Time ATP rate assay Test Kit can be used to characterize the cell line/type of interest in a single assay. To effectively examine metabolic and bioenergetic function using your Agilent Seahorse Extracellular Flux Analyzer, Analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal and maximal respiration (OCR) and extracellular acidification (ECAR). Support Services, Agreements & Trainingīasic procedures for choosing cell seeding densities.

Shorten the time it takes to start seeing the full value of your instrument investment Instrument & equipment deinstallation, transportation, and reinstallationĬrossLab Connect services use laboratory data to improve control and decision-makingĪdvance lab operations with lab-wide services, asset management, relocation Learn essential lab skills and enhance your workflows Instrument/software qualifications, consulting, and data integrity validations Software to manage instrument access/usage, sample processing, inventories, and more Service Plans, On Demand Repair, Preventive Maintenance, and Service Center Repair

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